Download e-book for kindle: Active Transport through Animal Cell Membranes by P.G. LeFevre

By P.G. LeFevre

ISBN-10: 3211803874

ISBN-13: 9783211803875

ISBN-10: 3709157684

ISBN-13: 9783709157688

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Ketogluterate, 5 mM. . and incubated 15 min. at aerobically . anaerobically . . . 1 0° C.. . :Wu C. . Na+ is here removed from medium, while almost none is lost from particles. "Cyclophorase" preparations all incubated 30 minutes at 20°C. 5 mM. ~aHC03 STANBURY and MUDGE (1953) attempted to analyse the relation of the mitochondrial K+ maintenance to the metabolic activity in similar preparations from rabbit liver. ; upsets in pH or osmotic pressure had to be rather extreme to affect the K+ accumulation, but ultimately caused a depression which paralleled the drop in the oxygen consumption.

O ... E"; go! 0 ° - ,, - - - ,, - - - - - - ) ( 'I. 0 30 60 TI_ (.. J 0 120 Fig. 8. Accumulation of K and glutamic acid by washed Staph. ~. Cells incubated (5 mg. ) for 1 hr. 0, with or without 2% glucose and 5 micromolar glutamic acid. Solid lines - K+; broken lines - glutamic acid. Open circles - with glucose only; crosses - with glutamic acid only; solid dots - with both. (Courtesy of Biochemical Journal) DAVIES et al. (1953), studying primarily the uptake of amino acids by microorganisms (which will be taken up in a later section) observed that this process often involves an accompanying shift in cations.

Fig. : P. G. LEFEVRE, Active Transport through Animal Cell Membranes for example the entrance of K + accompanying the glucose-stimulated uptake of glutamic acid by Staphylococcus aureus. Note that glucose in itself also initiates a lesser degree of K + uptake. These phenomena were observed also in Streptococcus faecalis and Saccharomyces fragilis; the incremental K+ entry never exceeded the amino acid uptake, mol for mol. The yeast also took in Na+ as well as K +, and Staphylococcus would do so only in the absence of K +.

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Active Transport through Animal Cell Membranes by P.G. LeFevre


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