Analysis of Sterols and Other Biologically Significant by W. David Nes PDF

By W. David Nes

ISBN-10: 0125154453

ISBN-13: 9780125154451

Research of Sterols and different Biologically major Steroids offers the basic education for the research of chosen sterols and steroids.

The e-book consists of chapters that evaluate the spectroscopic and chromatographic homes of sure sterols and steroids. The textual content additionally teaches the best way to isolate and represent sterols and steroid metabolites of plant, fungal, and bug beginning. Lipoprotein research and the usage of physical-analytical options are likewise provided.

Biochemists, microbiologists, and clinical physiologists will locate the e-book worthy.

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1963). Factice chromatography: An automatically monitored, liquid-gel system for the separation of nonpolar lipids. /. Lipid Res. 4, 1—10. , Jr. (1958). The separation of complex lipide mixtures by the use of silicic acid chromatography. J. Biol. Chem. 233, 311-320. , and Stabursvik, A. (1986). (22R)-22-hydroxycholesteryl esters from Narthecium ossifragum. Phytochemistry 25, 759-760. Jacks, T. , Yatsu, L. , and Altschul, A. M. (1967). Isolation and chracterization of peanut spherosomes. Plant Physiol.

The extraction system involving hexane and an aqueous salt phase is quicker and much easier to separate than the systems previously described. The extraction solvent containing the lipids is typically dried by rotary evaporation under vacuum at 15°C or less or by a stream of nitrogen gas under low heat. Solvents are usually redistilled prior to use to remove impurities and peroxides that may cause auto-oxidation of double bonds in the sterol or fatty acid. The pH of the steryl ester environment should be less than 7 to avoid hydrolysis of the ester bond.

20, 681—685. Tsai, L. , and Hudson, C. A. (1981). High performance liquid chromatography of oxygenated cholesterols and related compounds. /. Am. Oil Chem. Soc. 931-934. Van Lier, J. , and Smith, L. L. (1968). Crystalline sterols obtained by gas chromatography. /. Chromatogr. 36, 7-17. Volkman, J. , Gagosian, R. , and Wakeman, S. G. (1984). Free and esterihed sterols of marine dinoflagellate Gonyaulax polygramme. Lipids 19, 457—464. Vroman, H. , and Cohen, C. F. (1967). Separation of sterol acetates by column and thin layer argentation chromatography.

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Analysis of Sterols and Other Biologically Significant Steroids by W. David Nes

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